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Fig. 4. Construction and screening of tailored… Fig. 6. CRISPRi screening and characterization of… Synthesis, spectral characterization and biological research of some organotin(IV) complexes of L-proline, trans-hydroxy-L-proline and L-glutamine. Early research revealed that rooster embryos incubated with a rare analog of l-proline, 4-oxo-l-proline, confirmed elevated levels of the metabolite 4-hydroxy-l-proline. In 1962, 4-oxo-l-proline reductase, an enzyme answerable for the reduction of 4-oxo-l-proline, was partially purified from rabbit kidneys and characterized biochemically. We conclude that BDH2 is a mammalian 4-oxo-l-proline reductase that converts 4-oxo-l-proline to cis-4-hydroxy-l-proline and to not trans-4-hydroxy-l-proline, as initially thought. We additionally hypothesize that this enzyme may be a possible source of cis-4-hydroxy-l-proline in mammalian tissues. The actin regulator profilin 1 is functionally associated with the mammalian centrosome. Fig. 3. Codon saturation mutagenesis of γ-glutamyl kinase of C. glutamicum (CgProB) for improved l… Fig. 5. Enhancing l -proline production by… Fig. 5. Enhancing l -proline manufacturing by tuning the expression of flux-management genes. Fig. 3. Codon saturation mutagenesis of γ-glutamyl… Development of a CRISPR/Cas9 genome enhancing toolbox for Corynebacterium glutamicum. Sustainable and high-level microbial manufacturing of plant hemoglobin in Corynebacterium glutamicum. Metabolic engineering of Corynebacterium glutamicum for L-arginine production. The Escherichia coli high-quality amino acids manufacturer Acid Uptake Protein CycA: Regulation of Its Synthesis and Practical Application in l-Isoleucine Production.